Thứ Năm, 30 tháng 8, 2018

News on Youtube Aug 30 2018

If you're like most people, you don't want to read instructions.

Nobody wants to read instructions.

But, if you're going to do government contracts, there are instructions and you're going to

need to know how to use them.

I'm going to give you a five minute, laymen's terms: How to use government instructions,

the Instructions to Offeror is what it's called, 52.212-1 through 52.212-5.

It's called Instructions to Offeror and we're going to cover that now.

These rules typically apply to most government opportunities.

Purchasing agents in most case are looking for a reason to throw opportunities out.

And not following the instructions can get your bid or proposal or solicitation

thrown out without even reviewing it.

Now, imagine this the purchasing agent receives five or six offers and they only need three.

You follow the instructions, but two or three of the other people don't. Their proposals

are getting thrown out.

There's half your competition gone right off the bat.

So 52.212-1, Instructions to Offeror is basically how the government wants you to submit your

bid, what documentation they want you to include.

You can see it starts out with the North American Industry Classification System NAICS code

and small business size standard.

In other words, whatever NAICS code the purchasing agent is using, there's a size standard for

that NAICS code based on number of employees or revenues from last year or average three-years

revenues.

They'll specify which NAICS code and there's a size standards chart.

If you need it, let me know, I can send you a copy of it.

And that size standard basically certifies you as a small business.

Submission of offers is typically - they're either going to give you a detailed description

on how they want it submitted or they're going to reference 52.212-1, Instructions to Offerors.

It's really a simple requirement for submitting bids.

Based on the requirements that they're looking for they're usually asking for a cover page,

signature page, acknowledge of amendments page, sometimes the acknowledge of amendments

will be on the standard form, sometimes there's a spot on the standard form that you would

have on the cover or the signature.

I suggest, unless they specify, that you don't always include a signature page with a wet signature.

52.212-2 is how they're going to evaluate your bid, proposal or solicitation.

If it's a small business set-aside- it's rarely about price when it's a small business set-aside.

And it will say it right in the synopsis that the most important factor is your solution.

Your technical proposal is the most important factor.

Your technical proposal, if it's a widget is just a brochure for that widget guaranteeing

that it's exactly the model and part number that they want.

Or if it's a service your technical proposal is a detailed description on how you are going

to provide that service.

The technical proposal should mirror the Statement of Work or the Performance Work Statement.

If they're buying a widget, then the statement of work is simply that part and model number.

If it's a service, then your technical proposal is a detailed description on how you're going

to provide the service based on the information on the Statement of Work or the Performance

Work Statement, how you're going to meet or exceed the purchasing officer's expectations

is basically what the technical proposal is.

So, on small business set-asides your technical proposal is the most important factor, your

solution to their problem is the most important factor.

Your past performance is second.

Your price is last.

99.9% of the time it's not about price, it's about solution.

Full and open competition is just the opposite.

Full and open competition assumes that everyone has the same solution, therefore it's about

price and it's about experience.

But, that's 52.212-2, Instructions to Offeror in a nutshell is how the government will evaluate

your solution.

52.212-3 is basically- you just download your Reps and Certs right off of SAM.

And we process it for you so all you have to do is download it and save it and you're

going to include that document with your proposal in most cases.

52.212-4 and 5 are really just the legality of working with the government.

It varies per contract, but it's the red tape.

It's the terms and conditions if you will of working with the government.

And it's making sure that the government covers their rear.

Here's the thing guys, this is not a one time learn and done.

You need to continuously learn when it comes to government contracting.

It's like most things.

You know, if you're a doctor, you do continuing education.

You learn constantly.

I've been doing this since 2009.

I learn new stuff every week and the government's always changing.

It's a never-ending process, but if you really want to be successful with the government,

you need to learn and you need to continue to learn.

And that's what the training's for.

Get on one of my live trainings.

Check it out.

And continue to learn.

We've narrowed what you need to know down to the basics to make it easy and simple to

win contracts.

John Wayne, signing off.

Make sure to like and subscribe and share.

If you have not subscribed to the USFCR Youtube channel, you need to do that now.

Not tomorrow, not today, to-now because we are posting all my new media on the USFCR

Youtube channel.

Alright. Thanks guys. Bye.

For more infomation >> 5 Simple Instructions Required for Most Government Contracts - Duration: 5:11.

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3 Simple Mindset Shifts to Get More Clients - Duration: 7:49.

What's up rule breaker?

Welcome back to my channel.

In this video I'm going to talk to you about the three key mindset shifts you can make

to double your sales and get twice as many clients.

The farther I get in business, the more it becomes clear that mindset is everything.

The reason you don't have what you want right now is because you don't believe it's possible

and everything you have right now is a result of your past beliefs.

So I don't know if you know this, but I run a program called EA for clients where I coach

entrepreneurs like you to create signature services and book out those services completely

so that they can make a consistent full time income from their business.

Now, we've had more than 150 students go through this course and through the last year or two,

I've been able to see a few patterns in the entrepreneurs who are successful and those

who struggle a little bit more.

It's not about your God given talent.

It's not about being cut up for success.

It's not about how much money or resources you have.

Resourcefulness is more important than resources by the way.

It's not about how old you are, how young you are.

It's not about where you live or your first language.

It has nothing to do with any of those things.

The people who are more successful are those who get in the right mindset and specifically

there are three mindset shifts that they're able to make in order to start getting those

clients coming to them on a regular basis and truly becoming a boss of their own business.

And that brings me to our first mindset shift, which is become a boss, not a freelancer.

There is a massive difference between the mindset of someone who is freelancing or basically

just being reactive to what gigs are coming their way and competing for opportunities

versus a boss was running their own business, deciding what services or products they're

going to offer, and is selling those products and growing that business.

Huge, massive difference.

We all start off, or most of us start off as freelancers, but at a certain point, once

you realize what industry you want to get into, you need to transition from being a

freelancer to being a boss.

This is going to require that you decide how your business is run, not your clients.

That you decide it in advance who you want to work with, not react to the opportunities

out there.

And that you create a signature offer that you're going to sell again and again and again

rather than creating custom proposals or customizing your services to the client.

I know that sounds crazy, but it actually helps you to get more clients at higher price

points and get more work done in less time.

So it's a pretty great mindset shift to make.

So do you need to decide that you're a boss?

If so, I want you to put "boss" in the comments below.

That's going to be your first step towards making this mindset shift.

The second mindset shift you've got to make is to start seeing selling as service.

Here's what happens to a lot of my students and other entrepreneurs.

They create a service, they go through the process that I talked about in step number

one.

They start seeing themselves as a boss instead of a freelancer, and then when it comes to

actually asking for money for what they do, they freak the eff out.

A lot of unresolved worries about unworthiness and money and limiting beliefs come into play

here.

The big thing is seeing selling as a negative versus a positive.

I've used selling as adding a net benefit to the world as a whole, and I always use

selling as a win-win situation for me and my client.

Ultimately I'm helping the person to get a result they desire and there's a price tag

on that and one thing I really want you to remember is that people who pay to play get

better results than freebie chasers.

I've had people who have watched my videos and consume my content for years and have

never taken any steps forward or made progress because they've never invested in themselves.

Now, I'm not saying that they need to invest with me, but I can see that this has become

a pattern of "I want more and more and more free content."

And that's fine and I love creating free content and making my trainings accessible to people.

However, until you really step up and make an investment, there's only so far you're

going to go.

No business owner has made it without ever investing in themselves.

By charging good prices for what you do, you are being of service to your client.

You're going to do better work, your client's gonna respect you more, and everybody's going

to get a better result in the end.

Now, if you're still struggling with this, that means we need to address a mindset shift

number three, which is viewing money as a limitless.

We are in a universe of limitless abundance, and if you're feeling like you're stealing

from people by asking them to pay for what you do, you have a lack mentality.

Totally fine, I also fall into this from time to time.

There is truly no limit to how much money exists in the world.

The only limit is the limit you're putting on yourself.

Now, this one's really hard sometimes because we've been so ingrained in the trading hours

for dollars scheme and viewing it as a having a cap on your income and it's just not true.

The biggest step here is to step out of that trading hours for dollars or looking at your

prices as an hourly wage situation.

So let me give you an example.

Let's say that you do coaching and in your coaching package you have a monthly call with

people for three months, so that's three calls, three hours of calls, and you decide that

you're going to base your pricing around the fact that there's three hours of calls that

that person is paying for and you think, oh well, I can only really charge $100 an hour,

so the max amount I can charge for my three month coaching program is $300.

When you price your services based on the hours of work you are doing yourself and your

clients a massive disservice.

Instead you need to look at pricing it based on the value that you're creating for someone.

Which also means that you can add other elements to your services other than just those hourly

calls, because let's face it, there's also prep time and post call time and organization

and an administrative work that has to happen to make those calls happen as well.

But in order to get somebody to pay you for that, they don't care how many hours you're

spending prepping for the call.

What they want to know is what is the net benefit to them?

In other words, what is their return on investment?

This doesn't necessarily have to be financial, however, I'm going to challenge you if you

think that your services don't have a financial benefit, even if it's not a direct benefit.

But also think about the other benefits of your service.

How is your client's life or business going to change as a result of working with you

and what is that worth to them?

Now, these three mindset shifts are going to get you willing to take the actions you

need to take in order to get results.

So once you've made the mindset shifts, then it's time to get your butt in gear and start

taking action.

Now, I've actually got a really great freebie for you, which is a checklist of 64 actions

that you can take to start booking more clients and book out Your business.

So if you'd like to grab your copy of that, just go ahead and scroll down to the description

box below this video and grab your copy.

I would love to know which one of these mindset shifts is the most important for you.

I already said, if you need to start seeing yourself as a boss instead of a freelancer,

write "boss" in the comments.

But I'd also love to know, do you need to start seeing selling as service?

Well then I'd love you to write "selling is service" in the comments.

Let's create an affirmation around that.

And if you need to start seeing money as a limitless, why don't you write that in the

comments below?

I can't wait to cheer you on.

And don't forget that if you liked this video, to subscribe to my channel because I publish

videos like this one every single week for you.

Go ahead and hit the bell so you'll get notified when the next video comes live.

And finally, please hit that like button so that you can tell YouTube that this video

was amazing.

Thank you so much again for watching and I'll see you next time.

For more infomation >> 3 Simple Mindset Shifts to Get More Clients - Duration: 7:49.

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Machine Learning #3 - Simple Test Using Tree Decision - Duration: 5:28.

to be able to master a science, learning is the key.

especially in the IT field

but for certain cases, reading alone is not enough.

But you have to practice too so you can understand.

and in this case

we will try the existing machine learning platform

the goal is that we can understand first with the example of this existing framework

in this video, we will try scikit-learn

for the link in the description

according to the data we have classified in the previous video

we already have 4 features and labels

For its features, it consists of IP Address, URL address requested, Useragent, and respond to the code.

If we implement this data into codes

it will be like this

In feature variables, there are values that we have taken from logs

While on the label variable,

each index represents the status of the request from each index array in the features

the feature will be labeled OK, if the request made by the user is normal

and

NOT OK

if the request made by the user is suspicious

Because the value calculated by this bit is in the form of numbers

so we change each value from the feature array to a number

For the IP Adress key in the array, we will only give values 0 and 1

Zero, if the IP of the user making the request is not listed in the blacklist

and One, if the IP of the user making the request is included in the blacklist

In this case, this is because we only analyze local logs

so we assume that all IP values are 0

That is, IPs that are not included in the blacklist

Then in the part of the requested URL address

we will only give values of 0 and 1

* sound of a rooster crowing *

what a rooster -_-

Zero, if the requested URL is not included in the whitelist

and 1 if the requested link is the permitted links

For the discussion on how to make this URL whitelist

I have discussed in the previous video with the title "Creating a Web Crawler From Scratch"

the link can be seen in the description.

In the "useragent" key, we will specify the code manually

For example number 1 for Firefox, 2 for Chrome, 3 for Opera, and 4 for UserAgent besides that.

And finally the key "response code"

because the value is already in the form of numbers, so we don't need to change it.

After classification and simplification of data is complete

next we will include scikit-learn

to training the new data

using Tree Decision method

For documentation can be read directly on the scikit-learn website

link in the description

this is the declaration of the method we use

and this is the training process

then we will detect whether the request from the user is

OK or NOT OK

we use predict

as example, the IP Address is in the whitelist

the URL is in whitelist too

using Firefox browser

and respond code 200

this an example of Normal Request

now we run this simple test

we managed to get the OK output

now we try the opposite

useragent is curl, so it's code is 4 (else)

the requested URL is not in the whitelist

respond code 404

now run

NOT OK

last we tried to combine OK and NOT OK conditions

the IP is still

as whitelist

requested URL

is not in the whitelist

using Firefox

and respond code 200

now run

for accurate results

depends on

trained data

so, what do you think guys? easy isn't it?

!@^#$@#!

If you are still curious or for those of you who want to share about machine learning

please directly join in the telegram group

the link is available in the description

see you in the next video

ciaoo

For more infomation >> Machine Learning #3 - Simple Test Using Tree Decision - Duration: 5:28.

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🔴 DIY simple BALSA PLANE for KIDS / BEGINNERS - Duration: 4:46.

Hi.

Today we will make a simple plane from balsa.

Welcome to my channel GreenEyedExplorer, where I explore further and beyond about tweaks,

hacks, electronics and house repairs.

Before we start let's see how the plane flies.

At the end we reveal some tricks to make the plane more durable.

THINGS YOU WILL NEED

cutting mat

balsa square strip

balsa sheet

wood glue

sharp knife or scalpel

ruler

toothpicks

some Lego blocks

modelling clay

pencil

CUTTING PLANE PARTS

First we will cut two wings.

Each 18x3cm or 7x1.2".

First measure 18cm on top and then on the bottom.

Mark the line inbetween.

Secondly, mark two-times 3cm on both sides and gently cut out the wings.

Secondly we will cut the horozontal stabilizer.

That is tail wing.

Only one.

First we will mark 9cm on the top an the bottom and a line inbetween.

Then mark 2cm width on both sides and cut out the tail wing.

Next we will cut out the plane body from balsa

square strip.

Mark the 25cm line all around the strip and gently cut it to prevent it from tearing.

Now we will cut the vertical stabilizer.

That is tail of the plane.

Here are the measurements.

It has a specific shape.

Because this is our second plane, we already have the angled cut-out.

The animation shows how to cut the first and the second tail.

GLUING THE PLANE GLUING WINGS

Now we will start gluing parts together.

Take your plane body and a ruler.

See the marks on the screen where and what to measure.

Now mark the starting point of the wings from the plane nose.

Mark at 11cm (4.3").

Next prepare your Lego blocks.

Put 3 together for each wing.

Now put wood glue to each wing and glue them behind the mark we have made on the body.

When the glue dries, put some more glue around the joint.

On the bottom and on the top.

GLUING TAIL WING Let's glue the tail wing.

Measure and mark the middle of the tail wing.

All the way around.

Now put some glue on the line and join the tail and the body.

Now we will the tail.

Prepare two additional Lego blocks as seen on the screen.

See how it's made upclose.

TIPS

TIP #1

When choosing a knife, always use a sharp one to avoid tearing of the balsa.

TIP #2 Here you can see how to make the wing joint

sturdier.

Use some balsa leftovers, cut them to wing width and glue them to the top.

It is easier if you first apply the glue and push sticks into it.

Here you can see details.

TIP #3 Tail will break the first.

We advise you to fasten it with a toothpick.

Cut it to appropriate length and glue it to the back of the tail.

TIP #4 As balsa is very thin and lightweight we advise

you to always cut it toward the middle to prevent is from splitting.

For the same reason have your knife sharp and always cut at a low angle.

BALANSING THE PLANE Before the first flight you will have to balanse

the plane.

Use some modelling clay and put it on the nose of the plane.

The amount is correct when the plane does not tilt.

If you feel we have contributed to your knowledge,

don't hesistate to buy us a juice.

The link is below.

You can even support us monthly via PATREON.

And please do hit the LIKE button, SHARE the video with your friends, SUBSCRIBE to our

channel and COMMENT below what adjustments you have made.

See you next time!

For more infomation >> 🔴 DIY simple BALSA PLANE for KIDS / BEGINNERS - Duration: 4:46.

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mehndi designs 2018 new style | easy and simple | Marriage mehandi | front | back | Bride full hands - Duration: 1:02.

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For more infomation >> mehndi designs 2018 new style | easy and simple | Marriage mehandi | front | back | Bride full hands - Duration: 1:02.

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Photo Color Correction - Three Simple Ways to Fix Bleak Colors - Duration: 2:50.

Can't cope with your camera settings and get dull and lifeless photos?

Don't worry, you can restore their vivid colors in PhotoWorks software even if you're

an absolute newbie to image editing.

The easiest and fastest way to fix color and lighting problems is to use auto correction.

Open your photo in the program.

While on the Enhancement tab, check the Color box and the program will automatically saturate

all the colors.

Click View Source to compare the initial picture with the edited version.

If you took a photo in low light conditions and it came out too dark, tick the Light box.

The auto correction module will adjust exposure, contrast, highlights and shadows, so you won't

have to change any settings with your own hands.

For more subtle editing, it's better to adjust the color settings manually.

Switch to the Colors tab and drag sliders right or left to balance the tones of your photo.

That way you'll be able to bring out the chosen hues and increase the overall lighting.

Want to get a flawless photo?

Start working with Tone Curve like pro photographers do!

Type in Curves in the search bar and the program will open the tool for you.

Basically, there are four channels you can adjust - RGB, Red, Green and Blue.

If you intensify one of the channels, you subdue the opposite one.

For example, when you drag the Red Curve down it adds Cyan and when you drag the Red Curve

up it adds Red.

It works the same way for Blue and Yellow, Green and Magenta.

This shot looks too reddish for a winter photograph.

Switch to the Red channel and drag the curve downwards until the tones of the photo become colder.

Now, let's increase the overall contrast.

Switch back to the RGB channel and create a slight 'S' curve to accentuate both

dark and light areas.

Want to apply these tricks to your own pictures?

For more infomation >> Photo Color Correction - Three Simple Ways to Fix Bleak Colors - Duration: 2:50.

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Aroma Rice pressure cooker Receipe how to make Aroma Rice simple easy to make - Duration: 3:09.

For more infomation >> Aroma Rice pressure cooker Receipe how to make Aroma Rice simple easy to make - Duration: 3:09.

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( FREE 18 LUTS ) Simple how to use luts cinematic color grading with adobe premiere pro - Duration: 4:40.

hello everyone,

this time I will bring a simple tutorial how to use luts cinematic color grading with adobe premiere pro

ok first just go to premiere pro,

then import the video,

then input to the timeline

like this it's the initial display that hasn't used luts

then enter effects,

click the lumetri color tab,

click the creative tab

then extract the package luts first,

for the download luts link can be seen in the description below

ok you can see there are 18 luts available,

then go back to premiere pro,

click looks,

and select browse,

then select one of the luts that you want to use

like this it looks

then try another one,

like this

ok then try the second video,

and like this

then if it's still lacking,

can be set saturation, vibrance, sharpen, faded film, and intensity, like this

For more infomation >> ( FREE 18 LUTS ) Simple how to use luts cinematic color grading with adobe premiere pro - Duration: 4:40.

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new sravana masam muggulu | Simple chukkalu rangoli | Latest melikalu with dots | easy kolam designs - Duration: 1:37.

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For more infomation >> new sravana masam muggulu | Simple chukkalu rangoli | Latest melikalu with dots | easy kolam designs - Duration: 1:37.

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Простая роспись кошелька (eng sub) Simple painting of the wallet - Duration: 5:53.

Hello!

I'm glad that you went to the channel TessaArt!

Today we will do not difficult, but for the beautiful painting

purse.

List of materials in the description.

For the beginning I advise to degrease surface, as well as choose

smooth, one-color wallets, so it will be easier for you to work.

Next, you need a blue and white acrylic, as well as flat

synthetic brush.

We recruit one half Brush white paint, and on

second blue.

And making no complex movement we draw a petal

behind the petal.

The wallet is twisted for convenience.

Do not forget to press the bell to receive notifications

about new videos, and stop by in the "community" section on the

my channel.

I also advise you to look in my instagram there are published

Exclusive photos and videos materials.

Come and see everything yourself, all links in the description.

Then we take the blue color and we do all the same,

only with blue and blue.

After the first layer of flowers not bright enough and shine through.

So let's dry the layer and we put two more.

And when flowers suit us go to the greens.

We take green and yellow colors.

By the same principle on the part brushes type green

color, and the second part yellow.

And draw the leaves.

They will also have to be applied in several layers with an intermediate

by drying.

If you do not want to long wait, then you can take advantage of

hairdryer.

Just direct the streams air along the surface.

In the centers of flowers you can add a little yellow.

In the end, you just need to cover surface varnish to

the flowers served for a long time.

Here is a purse with us came out, it will be beautiful

a handmade gift.

Thanks for watching, I hope did you like it.

Put your finger up, sign on the channel and instagram.

Share photos of your works in the Vkontakte group.

Do not miss the new videos, bye Bye!

For more infomation >> Простая роспись кошелька (eng sub) Simple painting of the wallet - Duration: 5:53.

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Simple Free energy generator 220V DC motor with Light Bulb easy at School - Duration: 11:15.

Simple Free energy generator 220V DC motor with Light Bulb easy at School

For more infomation >> Simple Free energy generator 220V DC motor with Light Bulb easy at School - Duration: 11:15.

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What's Your Favorite Color? + More | Super Simple Songs - Duration: 56:00.

(lively music)

♪ My favorite color's blue ♪

♪ How about you, how about you ♪

♪ My favorite color's blue ♪

♪ How about you, how about you ♪

♪ Red ♪

♪ My favorite color's red ♪

♪ I like red, I like red ♪

♪ My favorite color's red ♪

♪ I like red, I like red ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

♪ Green ♪

♪ My favorite color's green ♪

♪ I like green, I like green ♪

♪ My favorite color's green ♪

♪ I like green, I like green ♪

♪ Yellow ♪

♪ My favorite color's yellow ♪

♪ I like yellow, I like yellow ♪

♪ My favorite color's yellow ♪

♪ I like yellow, I like yellow ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

♪ Orange ♪

♪ My favorite color's orange ♪

♪ I like orange, I like orange ♪

♪ My favorite color's orange ♪

♪ I like orange, I like orange ♪

♪ Purple ♪

♪ My favorite color's purple ♪

♪ I like purple, I like purple ♪

♪ My favorite color's purple ♪

♪ I like purple, I like purple ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

♪ Colors, colors ♪

♪ What's your favorite color ♪

For more infomation >> What's Your Favorite Color? + More | Super Simple Songs - Duration: 56:00.

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はみがきのうた | こどものうた | Super Simple 日本語 - Duration: 2:15.

Brush your teeth up and down

Brush your teeth round and round

Brush your teeth from left to right

Brush your teeth in the morning and night

Brush brush brush, Brush brush brush

Brush your teeth in the morning and night

Brush your teeth to keep them white

Brush your teeth so your smile is bright

Brush your teeth. It's so much fun

Brush your teeth when the day's begun

Brush brush brush, Brush brush brush

Brush your teeth when the day's begun

Brush your teeth, just open wide

Brush your teeth from side to side

Brush your teeth you sleepy head

Brush your teeth before you go to bed

Brush brush brush, Brush brush brush

Brush your teeth before you go to bed

Brush your teeth up and down

Brush your teeth round and round

Brush your teeth from left to right

Brush your teeth in the morning and night

Brush brush brush, Brush brush brush

Brush your teeth in the morning and night

For more infomation >> はみがきのうた | こどものうた | Super Simple 日本語 - Duration: 2:15.

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latest blouse designs with full sleeves,simple maggam work blouse designs | aari work,TNBN Tv Live - Duration: 6:16.

TNBN TV Live

For more infomation >> latest blouse designs with full sleeves,simple maggam work blouse designs | aari work,TNBN Tv Live - Duration: 6:16.

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Ce truc simple qui vous aidera à supprimer les points noirs une fois pour toute - Duration: 2:19.

For more infomation >> Ce truc simple qui vous aidera à supprimer les points noirs une fois pour toute - Duration: 2:19.

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A simple blood test could lead to better cancer treatment - Duration: 2:28.

Researchers have taken a significant step towards personalizing the treatment of cancer.

Using DNA sequencing, they've developed a way to scan blood samples for mutations

in circulating tumor DNA -- small bits of genetic material that are released as cancer

cells die.

The genomic reservoir contained in this DNA is representative of nearly all tumors carried

by a patient, providing the foundation needed for comprehensive genetic profiling.

Such profiling may help clinicians select the most appropriate therapies for a given

patient.

The genetic mutations that drive cancer development often differ markedly among individuals.

Interventions that match a patient's unique genetic profile offer great promise, but obtaining

tumor tissue for genetic testing can be invasive, risky and sometimes impossible.

To bypass these limitations, the researchers established a way to extract, sequence, and

analyze circulating tumor DNA.

The method, which relies on targeted and simultaneous sequencing of a panel of 382 cancer-relevant

genes, is more time and cost effective than traditional single-gene analysis.

The team used the approach to assess 605 patients with 29 different types of solid tumors.

The patients were divided into two groups.

In the first group, the researchers compared mutation profiles between circulating tumor

DNA and tumor specimens collected from the same patient.

Approximately 80% of these patients had abnormalities in circulating tumor DNA, and nearly 75% of

the abnormalities matched those found in the tumor tissues.

In the second group, the team tested only circulating tumor DNA.

The two groups showed similar types of mutations, suggesting that circulating tumor DNA is a

viable alternative for genetic profiling.

Even more encouragingly, among all the patients, 71% of the circulating tumor DNA samples contained

at least one clinically actionable mutation, and 66% of these could be targeted by drugs

already approved or currently in clinical trials.

These findings support that a simple blood test can help guide the personalized treatment

of cancer.

Because of the non-invasive nature of the testing, it can also be easily repeated.

This may allow clinicians to better monitor disease progression, potentially getting patients

the care they need faster.

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